关键词: 燃煤砷污染, DNA 合成, DNA 损伤及修复, 液体闪烁计数法, 125 I 后标记法

Abstract: Purpose and Methods :Using liquid scintillation count assay and 　I2postlabeling assay , DNA spontaneous synthesis、DNA2protein crosslinks (DPC) and unscheduled DNA synthesis (UDS) were detected to study DNA synthesis、repair function and DNA damage condition in human peripheral blood cells of arsenism caused by burning coal. Results : The DNA synthesis of non-patient s and patient s in local area obvious decreased , while DPC level increased with the development of poisoning , and UDS increased only in the patient s , the difference were all significant . Conclusion : It suggested that arsenic containing in coal might induce DPC in early stage and severely hurt DNA ; and it might induce UDS , the DNA synthesis and repair ability were seriously rest rained. It might be one of the reasons causing dermal cancer.

Key words: arsenic pollution, DNA synthesis, DNA damage and repair, Liquid scintillation count assay, 125　I-postlabeling assay